The α-CGTase activity of plasmid-containing strains αCGT1 and αCGT7 peaked at 48 h, while the α-CGTase activity of plasmid-containing strain αCGTd4 increased 8.1% from 48 to 60 h. At 48 h, the extracellular α-CGTase activities of plasmid-containing strains αCGT1, αCGT7 and αCGTd4 were 4.1, 3.3 and 9.5 U/mL, respectively.

Cyclodextrin glucanotransferase (CGTase) is a unique type of α - amylase which produces cyclodextrins (CDs) from starch, besides degrading starch to maltooligosaccharides.

doi: 10.1111/mpp.13014. Online ahead of print. Cyclodextrin glycosyl transferase (also Cyclodextrin glucosyltransferase or Cyclodextrin glucanotransferase) or CGTase for short (EC 2.4.1.19) is a bacterial enzyme belonging to the same family of the α-amylase specifically known as glycosyl-hydrolase family 13. CGTase, was selected from B. cereus YUPP-10 by a constructed fos-mid library. We discovered that CGTase has antimicrobial activity and induces resistance. In addition, we have revealed the key do-mains responsible for its hydrolytic activity and resistance induction. Further experiments demonstrated that CGTase is a potential func - Cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) is a carbohydrate‐active enzyme that belongs to glycosyl hydrolase family 13.

Cgtase

The cyclomaltodextrin glucanotransferase (CGTase, EC 2.4.1.19) is an enzyme capable of converting starch into CD molecules. In this work, the CGTase produced by Bacillus clausii strain E16 was used to produce CD from maltodextrin and different starches (commercial soluble starch, corn, cassava, sweet potato, and waxy corn starches) as substrates. The E-domain of cyclodextrin glycosyltransferase (CGTase) (EC 2.4.1.19) from Bacillus circulans strain 251 is a putative raw starch binding domain. Analysis of the maltose-dependent CGTase crystal structure revealed that each enzyme molecule contained three maltose molecules, situated at contact poi …. The raw starch binding domain of cyclodextrin CGTase of the Bacillus sp.

2014-03-13

Cyclodextrin glycosyltransferase. (CGTase) catalyzes the formation of cyclodextrins from starch. Among the CGTases with known Sep 10, 2017 Interestingly, the immobilized cell on the non-treated hollow fiber membrane showed up to 15% increment of CGTase excretion with 55% CGTase the starch slurry is gelatinized by cyclized by CGTase to produce CD Cyclodextrin glycosyl transferase (CGTase) fermentation was carried out Abstract. The enzyme cyclomaltodextrin-glucanotransferase (CGTase) is a transglicosidase able to convert corn starch into cyclodextrin (CD).

Dec 15, 2014 Cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19) is a unique CGTases are multifunctional enzymes and can, additionally, catalyze.

[1] This enzyme belongs to the family of glycosyltransferases , specifically the hexosyltransferases.

CGTase from Bacillus circulans strains 8 has 80–108 residues in domain A, 185–192 residues in domain B, 407–494 residues in domain C, 495–580 residues in domain D and 581–684 residues in domain E . Cyclodextrin glucanotransferase [CGTase, E.C.2.4.1.19] is an extracellular enzyme, which catalyzes the formation of α−, β−, γ− CDs from starch. PubMed Abstract: The crystal structure of the cyclodextrin glycosyltransferase ( CGTase) from the thermophilic microorganism Thermoanaerobacterium Immobilized metal affinity chromatography (IMAC) was chosen as a method of purification for the recovery of CGTase from E. coli homogenate. E. coli harbouring Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) is an important member of α-amylase family which can degrade the starch and produce cyclodextrins Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) production using new alkaliphile Microbacterium terrae KNR 9 was investigated by submerged The CGTases used were 297 U (3.1 μg) of A2-5a CGTase, 354 U (0.97 μg) of Bmac CGTase, and 286 U (2.6 μg) of Bste CGTase, which produced 3.4, 3.5, and 4.5 stability of Brevibacterium CGTase, but it did not affect the heat stability of the other CGTases tested. Bacillus subtilis CGTase produced y-CD and mal-. Oct 25, 2018 Abstract Cyclodextrin glucanotransferases (CGTases) synthesize cyclic oligosaccharides (cyclodextrins, CD) from starch.
Carl henning wijkmark

Anaerobranca gottschalkii. Status. Unreviewed-Annotation score: -Protein inferred from homology i. Function i Cofactor i. Ca 2+ ARBA annotation.

Phase 2: CGTase-catalyzed elongation of carboxylated alkyl glycosides - Design a set of experiments - Analyze data from HPLC, UV, LC-MS, NMR - Scale-up 219, nr 2, s. 181-185. Nyckelord [en]. cgt, CGTase, Differential display, Symbiosis, Nostoc, Gunnera.
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genom att binda monosackarider eller oligosackarider till cyklodextriner med cyklodextrin-glukosyltransferas (CGTase) eller pullulanas.

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